pe antimouse cd80  (Proteintech)

Journal: Journal of nanobiotechnology

Article Title: Orchestrated Cu 2+ -coordinated tetracycline-porphyrin self-assembly remodels tumor microenvironment for photo-enhanced immuno-chemodynamic therapy.

doi: 10.1186/s12951-025-03486-9

Figure Lengend Snippet: Fig. 8 In vivo immunomodulatory effects of LP/CuTT. Representative flow cytometric analysis and quantification of tumor-infiltrating M1 TAMs gated on CD11b+ and CD80+ cells (A, B), CD4+ T cells gated on CD3+ and CD4+ cells (C, D), and CD8+ T cells gated on CD3+ and CD8a+ cells (E, F) from the upper right quadrant of the scatter diagrams in tumors after 17 days treatments. G-L) Representative flow cytometric analysis and quantification of M1 TAMs, CD4+ T cells and CD8+ T cells in spleen after different treatments. Serum levels of cytokines M) TNF-𝛼, N) IL-1β, and O) IL-10 in mice after different treat ments detected by ELISA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared to control. #p < 0.05, ns indicates no significant difference

Article Snippet: PE anti-mouse CD206/MMR antibody, FITC anti-mouse CD86 antibody, APC antimouse CD80 antibody, FITC anti-mouse CD3 antibody, PerCP anti-mouse CD8 antibody, and APC anti-mouse CD4 antibody were purchased from Elabscience (Wuhan, China).

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Control

Journal: Comparative Medicine

Article Title: Effect of Chronic Uremia on the Cell Surface Expression of B7 Family Costimulatory Molecules in an HLA-A2 Transgenic Mouse Model of Chronic Kidney Disease

doi:

Figure Lengend Snippet: Surgical induction of chronic renal failure diminishes CD86 expression in HLA-A2 mice. The frequency of CD80+ and CD86+ cells in sham-operated and CKD mice was determined at 13 wk after the nephrectomy or final sham procedure by flow cytometry analysis using splenocytes obtained by mechanical disruption of the spleen. (A) Representative histograms showing decrease in frequency of CD86+ spleen cells in CKD (right panel) compared with sham-surgery mice (left panel). The black line indicates the isotype control, and the red line indicates the CD86+ population. (B) The percentage of splenocytes expressing either CD80 or CD86 in sham compared with CKD animals §, P < 0.0001 (Mann–Whitney analysis).

Article Snippet: The cells were stained with antimouse CD80–PE conjugate (BD Pharmingen) or antimouse CD86–PE conjugate (clone P03.1, eBiosciences) monoclonal antibodies for 30 min at 4 °C.

Techniques: Expressing, Flow Cytometry, MANN-WHITNEY

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Carthamus tinctorius Enhances the Antitumor Activity of Dendritic Cell Vaccines via Polarization toward Th1 Cytokines and Increase of Cytotoxic T Lymphocytes

doi: 10.1093/ecam/nen068

Figure Lengend Snippet: CT extract enhances the maturation of mouse DCs. Mouse bone marrow cells were collected as described in . After 6 days of differentiation with GM-CSF and IL-4, the cells were treated with various concentrations of CT extract in the absence of GM-CSF and IL-4 for an additional 48 h. The cells were then scraped and collected for surface molecule analysis (CD80, CD86, MHC-I and MHC-II) using flow cytometry.

Article Snippet: Phycoerythrin (PE)-hamster antimouse CD80 (B7-1) (16-10A1 clone, Cat No. 553769), fluorescein isothiocyanate (FITC)-rat antimouse CD86 (B7-2) (GL1 clone, Cat. No. 553691), PE-mouse antimouse H-2K d (MHC-I) (SF1-1.1 clone, Cat. No. 553566) and FITC-mouse antimouse I-A d (MHC-II) (AMS-32.1 clone, Cat No. 553547) were purchased from Becton Dickinson-Pharmingen (NJ, USA).

Techniques: Flow Cytometry